MVSLIDE
IN NEUROANATOMY
mvSlide for digitizing brain sections
Light microscopic imaging of neuroanatomic slides not only requires high quality images, but frequently also involves large format slides or particularly thick specimens. As the Microvisioneer mvSlide whole slide imaging technology preserves the optical quality and the flexibility of the microscope, it is ideally suited for the acquisition of neuroanatomic digital images.
Example: Multiple sclerosis research study using mvSlide
In neuroanatomy research, mouse brains very often serve as experimental models to investigate various neurological diseases. In the study presented below, IHC-stained mouse brain sections were digitized using mvSlide.
In the referenced publication, mvSlide was referred to as manualWSI, the former name of the software.
In the recently published scientific study, performed by a research group around Prof. Markus Kipp and first author Stella Nyamoya, the potential therapeutic effects of the substance laquinimod on multiple sclerosis in mice were investigated using cuprizone-induced demyelination as model of multiple sclerosis.
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To assess the influence of laquinimod on remyelination on a molecular level, brain tissue sections were analyzed by immunohistochemistry following laquinimod treatment. Among others, anti-PLP staining was performed to indicate the presence of myelin. Whole slide images (WSI) were acquired using the mvSlide manual scanning software, and densitometric analyses of the intensity of the anti-PLP stainings were subsequently conducted in selected regions of interest (ROI).
Using mvSlide, high-quality whole slide images of stained mouse brain sections were generated with the existing microscope setup, without requiring investment in a substantially more expensive automated slide scanner. This enabled a fully digital and flexible workflow, including subsequent quantitative and representative image analysis.
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Below, representative whole slide images of anti-PLP stained brain sections are shown as examples.
Representative images of anti-PLP-stained mouse brain sections
Zoom into the scan using the mouse wheel to explore the different areas of the mouse brain sections from different treatment groups!
​Image gallery descriptions:
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Image (1): anti-PLP stained brain section of an animal assigned to a control group that has not been treated with cuprizone
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Image (2): anti-PLP stained brain section of an animal of the cuprizone-intoxicated group that has received a vehicle solution instead of laquinimod
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Image (3): anti-PLP stained brained section of an animal of the cuprizone-intoxicated group that has received the laquinimod treatment
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Images kindly provided by Prof. Markus Kipp and Ms M.Sc. Newshan Behrangi.
Analysis of the digitized brain sections revealed a significantly higher intensity of anti-PLP staining in the cuprizone-intoxicated group that has received the laquinimod treatment compared to the group having only received a vehicle. Together withthe additional data presented in the study, these results demonstrated that laquinimod treatment led to remyelination in the midline region of the corpus callosum in cuprizone-intoxicated mice.
Full study for all details and more information:
Stella Nyamoya, Julia Steinle, Uta Chrzanowski, Joel Kaye, Christoph Schmitz, Cordian Beyer, Markus Kipp: Laquinimod Supports Remyelination in Non-Supportive Environments. Cells, 2019
DOI: 10.3390/cells8111363 https://doi.org/10.3390/cells8111363
For further reading:
Stella Nyamoya, Patrizia Leopold, Birte Becker, Cordian Beyer, Fabian Hustadt, Christoph Schmitz, Anne Michel, Markus Kipp: G-Protein-Coupled Receptor Gpr17 Expression in Two Multiple Sclerosis Remyelination Models. Molecular Neurobiology, 2019
DOI:10.1007/s12035-018-1146-1 https://doi.org/10.1007/s12035-018-1146-1
CONTACT US
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83512 Wasserburg am Inn
Germany
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Tel.: +49 (0) 151 41605950
