Scanning at high-throughput with a manual device at first sight seems to be a contradiction. However, with the appropriate optical configuration, and the Microvisioneer manualWSI Professional Edition, even a higher volume of slides can be scanned manually at reasonable scan times.
How is it working?
Generally, the higher the selected objective lens magnification, the longer it takes to complete a scan. This is due to smaller fields of view (FOV) associated with higher magnification objectives.
However, per objective lens magnification, there are different levels of optical quality available, more specifically different levels of optical correction. Higher levels of correction result in a higher numerical aperture (N.A.), which in turn results in brighter images and better resolving power. The highest quality objectives are so-called apochromat objectives.
For high-throughput scanning, the use of such apochromat objectives in combination with appropriate cameras can significantly reduce the time required for scanning. If for a given scanning task 20X resolution is required, this can be achieved using a "standard" plan achromat objective, as well as with an apochromat 10X objective. As the apochromat objective provides a similar resolving power and at the same time has a larger FOV than the "standard" 20X objective, scanning will be significantly more efficient. Typical scan times for a "standard" configuration compared to a high-throughput configuration can be found in the table below.
What does it mean in practice?
Zoom into a representative image!
Objective Lens Magnification: 20X Quality with 10X APO (0.4 N.A.)
Tissue Size: 19x18 mm²
Scan Time: 6 min 50 sec
Microscope: Olympus BX60
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