HIGHER-
THROUGHPUT
MANUAL
SCANNING
Scanning at high-throughput with a manual system at first glance appears to be a contradiction due to the time required. However, with the appropriate optical configuration and the Microvisioneer mvSlide software, even higher slide volumes can be scanned manually within reasonable scan times - and affordable budgets.
How high-volume manual scanning works with mvSlide - Why optics matter
Generally, the higher the selected objective lens magnification, the longer it takes to complete a scan. This is due to smaller fields of view (FOV) associated with higher magnification objectives. However, objective lenses of the same nominal magnification differ in their level of optical correction and numerical aperture (N.A.).Higher levels of correction typically provide higher numerical apertures, resulting in improved resolving power and brighter images. Apochromat objectives represent the highest level of optical correction.
To fully benefit from the higher numerical aperture of apochromat objectives, the camera sensor (pixel size and sensor format) must be appropriately matched to the optical system.
For example, a required 20X resolution can be achieved either with a standard plan achromat 20X objective or with a high-quality apochromat 10X objective. Due to its larger field of view, the 10X apochromat can cover more area per image while maintaining comparable resolving power.
As a result, total scan time is significantly reduced. This improvement makes manual scanning with mvSlide a practical option even for higher slide volumes.
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In addition to the time-saving potential of the optical configuration, mvSlide also includes integrated features that improve workflow efficiency, in particular when scanning higher volumes: For example, a barcode scanner can be used to automatically read and create file names from barcoded labels, including regular expressions and time stamps.
Typical scan times for a "standard" configuration compared to a high-throughput configuration can be found in the table below.
Practical Example: Representative scan times
Desired Resolution
Scan time for 10x10 mm² [min]
Standard
High-Throughput
20X
40X
4.3
15.3
2.2 (with 10X apo)
7.3 (with 20X apo)
Zoom into a representative image!
​Scan Parameters:​
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Objective Lens Magnification: 20X Quality with 10X APO (0.4 N.A.)
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Tissue Size: 19x18 mm²
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Scan Time: 6 min 50 sec
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Microscope: Olympus BX60 ​
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CONTACT US
Microvisioneer GmbH
Schmidzeile 15
83512 Wasserburg am Inn
Germany
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Tel.: +49 (0) 151 41605950
